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:: Volume 8, Issue 3 (Fall 2010) ::
2010, 8(3): 193-199 Back to browse issues page
Molecular Epidemiology of Staphylococcus aureus Isolated from patients and personnel in Army hospital
N. Darabi , H. Habibollahi , K. Shahbabian
Corresponding Author), Tarbiat Modarres University, Research Center of 660 Nezaja, Tehran, Iran , ndarabi118@hotmail.com
Abstract:   (13851 Views)

Background: staphylococcus aureus has emerged over the past several decades as a leading cause of hospital- and community acquired infection. A significant component in the success has been its acquisition of antibiotic Resistant factors.We have developed a rapid and simplified approach for the strain characterization of staph.aureus on the basis of multilocussequence typing.

Method and material: MLRFT for s.aureus involes amplification of seven house keeping gene locus.the amplicons are then digested directly with one or two restriction Enzyme and the restriction fragments are resolved by agarose gel electrophoresis. These isolates had been additionally characterized for their susceptibilities to antibiotics.

Results: MLRFT resolved 146 isolates into 19 RFT that 48 isolates have the same molecular patterns. Their susceptibilities to antibiotics were showed 90% resistance to methicillin.

Conclusion: MLRFT provides convenient procedures for molecular epidemiology it requires minimal laboratory facilities and is relatively simple and inexpensive to perform.63% of cluster (24 isolates) take placed two fold cluster that 90% of them was resistant to methicillin, 55% belong to twofold cluster, there fore methicillin resistant isolates can transmit easily.

Keywords: staphylococcus aureus, Nosocomial infection, Restriction Enzyme, methicillin -resistance, MLRFT
Full-Text [PDF 590 kb]   (529 Downloads)    
Type of Study: Original (Research) | Subject: General Medicine
Received: 2011/05/3 | Accepted: 2016/10/3 | Published: 2016/10/3
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Darabi N, Habibollahi H, Shahbabian K. Molecular Epidemiology of Staphylococcus aureus Isolated from patients and personnel in Army hospital. Ann Mil Health Sci Res. 2010; 8 (3) :193-199
URL: http://journals.ajaums.ac.ir/article-1-603-en.html


Volume 8, Issue 3 (Fall 2010) Back to browse issues page
سالنامه پژوهش علوم سلامت و نظامی Ann Mil Health Sci Res
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