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:: Volume 7, Issue 3 (fall 2009) ::
2009, 7(3): 182-189 Back to browse issues page
Biological Control of Apple’s Blue Mold by Two Isolates of Saccharomyces Cerevisiae in Order to Reduce the Environmental Pollution
J Gholamnejad MSc , HR Etebarian PhD, MA Sheikh Beig Goharrizi PhD, A Nemati , F Naseri Nasab
, jalalgholamnejad2006@gmail.com
Abstract:   (9836 Views)
Background: In recent decays, application of the fungicides has provided some concerns in the scientific society. Recently, biological control was developed as an alternative to synthetic fungicides, and considerable success was achieved by utilizing antagonistic microorganisms for controlling postharvest diseases.
Materials and Methods: In this research, two yeast antagonists of Saccharomyces cerevisiae (04, 69) applied instead of fungicides that control blue mold of apple caused by Penicillium expansum. Blue mold agent was isolated from infected apples. Dual culture, cell free metabolite and volatile test were used in vitro assay to evaluate potential biological control agents against apple blue. In storage assays (in vivo assays), apple fruit wounds were inoculated with 40µl of yeast cell suspension (107cell/ml) followed 24 h later by P.expansum (105 conidia/ml). The apples were then incubated at 20°C and 5°C.
Results: The inhibition varied among isolates of yeasts and ranged from 33.12% to 58.4%, in dual culture, from 54.93% to 72.87% in volatile metabolite and from 50.23% to 76.23% in cell free metabolite test. In the storage test, two isolates of S. cerevisiae reduced the decay area from 1416 to 1435 mm2 compared to 3151.1 mm2 in control subjects at 5°C. At 20°C, the lesion area ranged from 1347 to 1598 mm2 for the antagonist treatments compared to 3257.4 mm2 for the control treatments.
Conclusion: The two isolates of S. cervisiae were effective at both temperatures and can be two important new biological control agents for apple blue mold.
Keywords: Saccharomyces cerevisiae, Penicillium expansum, Biocontrol, Volatile test, Cell free metabolite test
Full-Text [PDF 1929 kb]   (515 Downloads)    
Type of Study: Original (Research) | Subject: General Medicine
Received: 2010/04/13 | Accepted: 2016/10/3 | Published: 2016/10/3
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Gholamnejad J, Etebarian H, Sheikh Beig Goharrizi M, Nemati A, Naseri Nasab F. Biological Control of Apple’s Blue Mold by Two Isolates of Saccharomyces Cerevisiae in Order to Reduce the Environmental Pollution. Ann Mil Health Sci Res. 2009; 7 (3) :182-189
URL: http://journals.ajaums.ac.ir/article-1-467-en.html

Volume 7, Issue 3 (fall 2009) Back to browse issues page
سالنامه پژوهش علوم سلامت و نظامی Ann Mil Health Sci Res
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